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为探究黄河三角洲刺槐（Robinia pseudoacacia）枯梢与其根际土壤理化性质和土壤细菌群落结构的关系，以东营市河口区三大刺槐防护林（孤岛、军马五分场、九分场）为研究对象，分层采集健康和枯梢刺槐人工林根际土壤样品进行分析。结果表明：健康与枯梢刺槐根际土壤理化性质仅pH存在显著差异（P=0.015）。不同土层间细菌空间异质性显著，各层独有ASV占比达15%～27%；青枯菌属（Ralstonia）和假单胞菌属（Pseudomonas）在枯梢刺槐根际土壤中丰度显著下降，而硝化细菌（Nitrospira）在60～80cm土层相对丰度增加约10%；土壤细菌群落β多样性存在显著差异（P=0.016）。db-RDA显示pH是影响细菌群落结构的关键环境因子。刺槐根际土壤pH升高引起细菌群落改变，进而通过根际微环境导致枯梢现象，为黄河三角洲刺槐防护林的健康诊断与修复提供参考。
Abstract：To explore the relationship between dieback of Robinia pseudoacacia and its rhizosphere soil physicochemical characters and bacterial community structure in the Yellow River Delta,three major Robinia pseudoacacia shelter forests(Island,Warhorse Fifth Farm,and Ninth Farm)in Hekou District,Dongying City were selected as study sites.Rhizosphere soil samples from both healthy and dieback Robinia pseudoacacia protection forest were collected in five layers for testing.Comparative analysis revealed:Among the physicochemical characters of rhizosphere soil between healthy and dieback Robinia pseudoacacia,only pH showed significant differences(P=0.015).Bacterial spatial heterogeneity among different soil layers was significant,with unique ASVs(Amplicon Sequence Variants)in each layer accounting for 15%～27%.The abundance of Ralstoniaand Pseudomonas significantly decreased in the rhizosphere soil of dieback Robinia pseudoacacia,while the relative abundance of Nitrospira increased by approximately 10% in the 60～80 cm soil layer.Theβdiversity of soil bacterial communities showed significant differences(P=0.016).db-RDA indicated that pH was the key environmental factor influencing bacterial community structure.This study reveals that the increase in rhizosphere soil pH causes alterations in bacterial communities,and that the rhizosphere microenvironment triggers the dieback phenomenon,providing a scientific basis for health diagnosis and restoration of Robinia pseudoacacia protection forests in the Yellow River Delta.
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基本信息:
DOI：10.13486/j.issn.2097-4973.2025.06.015
中图分类号:S714.2;S792.99
引用信息:
[1]张勇,张军,王静,等.黄河三角洲刺槐防护林土壤理化性质和细菌群落研究[J].山东航空学院学报,2025,42(06):116-124.DOI:10.13486/j.issn.2097-4973.2025.06.015.
基金信息:
自然资源部长三角国土生态与土地利用野外科学观测研究站开放基金项目（2023YRDLELU06）; 滨州学院博士学位人员及具有硕士学位的高级职称人员科研启动费项目（2020Y22）
2025-12-15
2025-12-15